Publications

    2014

  • Yamashiro, S., Mizuno, H., Smith, M. B., Ryan, G. L., Kiuchi, T. Vavylonis, D. and Watanabe, N. New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales. Mol. Biol. Cell in press
  • Mizuno, H. and Watanabe, N. Rotational movement of formins evaluated by using single-molecule fluorescence polarization. Meth Enzymol. in press (2014)
  • 2013

  • Watanabe, N., Yamashiro, S., Vavylonis, D. and Kiuchi, T. Molecular viewing of actin polymerizing actions and beyond: Combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation). (Review) Dev. Growth Differ. 55, 508–514 (2013)
  • Higashida, C., Kiuchi, T., Akiba, Y., Mizuno, H., Maruoka, M., Narumiya, S., Mizuno, K. and Watanabe, N. F- and G-actin homeostasis regulates mechanosensitive actin nucleation by formins. Nat. Cell Biol. 15, 395-405 (2013)
  • Miyoshi, T. and Watanabe, N. Can filament treadmilling alone account for the F-actin turnover in lamellipodia? Cytoskeleton (Hoboken) 70: 179–190 (2013)
  • Smith, M.B., Kiuchi, T., Watanabe, N. and Vavylonis, D. Distributed actin turnover in the lamellipodium and FRAP kinetics. Biophys. J. 104: 247-257 (2013)
  • Ryan, G. L., Watanabe, N. and Vavylonis, D. Image analysis tools to quantify cell shape and protein dynamics near the leading edge. Cell Struct. Funct. 38: 1-7 (2013)
  • 2012

  • Koizumi, K., Takano, K., Kaneyasu, A., Watanabe-Takano, H., Tokuda, E., Abe, T., Watanabe, N., Takenawa, T. and Endo, T. RhoD activated by fibroblast growth factor induces cytoneme-like cellular protrusions through mDia3C. Mol. Biol. Cell 23:4647-4661 (2012)
  • Mizuno, H. and Watanabe, N. mDia1 and formins: screw cap of the actin filament. (Review) Biophysics, 8: 95-102 (2012)
  • Murata, K., Kitaori, T., Oishi, S., Watanabe, N., Yoshitomi, H., Tanida, S., Ishikawa, M., Kasahara, T., Shibuya, H., Fujii, N., Nagasawa, T., Nakamura, T. and Ito, H. Stromal cell-derived factor 1 regulates the actin organization of chondrocytes and chondrocyte hypertrophy. PLoS ONE 7: e37163 (2012)
  • Ryan, G. L., Petroccia, H. M., Watanabe, N. and Vavylonis, D. Excitable actin dynamics in lamellipodial protrusion and retraction. Biophys. J. 102: 1493-1502 (2012)
  • Ryan, G. L., Watanabe, N. and Vavylonis, D. A review of models of fluctuating protrusion and retraction patterns at the leading edge of motile cells. (Review) Cytoskeleton (Hoboken) 69: 195-206 (2012)
  • Millius, A., Watanabe, N. and Weiner, O.D. Diffusion, capture, and recycling of SCAR/WAVE and Arp2/3 complexes observed in cells with single-molecule imaging. J. Cell Sci. 125: 1165-1176 (2012)
  • Watanabe, N. Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms. Meth. Enzymol. 505, 219-232 (2012)
  • Sakamoto, S., Ishizaki, T., Okawa, K., Watanabe, S., Arakawa, A., Watanabe, N. and Narumiya, S. Liprin-α controls stress fiber formation by binding to mDia and regulating its membrane localization. J. Cell Sci. 125, 108-120 (2012)
  • Maruoka, M., Sato, M., Yuan, Y., Ichiba, M., Fujii, R., Ogawa, T., Ishida-Kitagawa, N., Takeya, T. and Watanabe, N. Abi-1-bridged tyrosine phosphorylation of VASP by Abelson kinase impairs association of VASP to focal adhesions and regulates leukemic cell adhesion. Biochem. J. 441, 889-899 (2012)
  • 2011

  • Sato, Y., Watanabe, N., Fukushima, N., Mita, S. and Hirata, T. Actin-independent behavior and membrane deformation exhibited by the four-transmembrane protein M6a. PLoS ONE 6, e26702 (2011)
  • Kiuchi, T., Nagai, T., Ohashi, K., Watanabe, N. and Mizuno, K. Live-cell imaging of G-actin dynamics using sequential FDAP. (review) Bioarchitecture 1, 1-5 (2011)
  • Smith, M.B., Karatekin, E., Gohlke, A., Mizuno, H., Watanabe, N. and Vavylonis, D. Interactive, computer-assisted tracking of speckle trajectories in fluorescence microscopy: application to actin polymerization and membrane fusion. Biophys. J. 101,1794-1804 (2011)
  • Kiuchi, T., Nagai, T., Ohashi, K. and Mizuno, K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension. J. Cell Biol., 193, 365-380 (2011)
  • Mizuno, H., Higashida, C., Yuan, Y., Ishizaki, T., Narumiya, S. and Watanabe, N. Rotational movement of the formin mDia1 along the double helical strand of an actin filament. Science, 331, 80-83 (2011)
  • 2010 and before

  • Watanabe N. Inside view of cell locomotion through single-molecule: fast F-/G-actin cycle and G-actin regulation of polymer restoration. (review) Proc. Jpn. Acad. B. Phys. Biol. Sci. 86: 62-83. (2010)
  • Narumiya, S. and Watanabe, N. Migration without a clutch. (News and Views) Nat. Cell Biol. 11: 1394-1396 (2009)
  • Monypenny, J., Zicha, D., Higashida, C., Oceguera-Yanez, F., Narumiya, S. and Watanabe, N. Cdc42 and Rac family GTPases regulate mode and speed but not direction of primary fibroblast migration during PDGF-dependent chemotaxis. Mol. Cell. Biol. 29: 2730-2747 (2009)
  • Tsuji, T., Miyoshi, T., Higashida, C., Narumiya, S. and Watanabe, N. An order of magnitude faster AIP1-associated actin disruption than nucleation by the Arp2/3 complex in lamellipodia. PLoS ONE 4: e4921 (2009)
  • Watanabe, N. and Higashida, C. A possible role of homeostasis between monomeric and filamentous actin in filament nucleation revealed by pharmacokinetic modeling. Systems Biology The Challenge of Complexity. Springer 143-149 (2009)
  • Fujita, A., Shishido, T., Yuan, Y., Inamoto, E., Narumiya, S. and Watanabe, N. Imatinib mesylate (STI571)-induced cell edge translocation of kinase-active and kinase-defective Abelson kinase: requirements of myristoylation and src homology 3 domain. Mol. Pharmacol. 75: 75-84 (2009)
  • Higashida, C., Suetsugu, S., Tsuji, T., Monypenny, J., Narumiya, S. and Watanabe, N. G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers. J. Cell Sci. 121: 3403-3412 (2008)
  • Shimada, T., Toriyama, M., Uemura, K., Kamiguchi, H., Sugiura, T., Watanabe, N., and Inagaki, N. Shootin1 interacts with actin retrograde flow and L1-CAM to promote axon outgrowth. J. Cell Biol. 181: 817-829 (2008)
  • Watanabe, S., Ando, Y., Yasuda, S., Hosoya, H., Watanabe, N., Ishizaki, T., and Narumiya, S. mDia2 induces the actin scaffold for the contractile ring and stabilizes its position during cytokinesis in NIH 3T3 cells. Mol. Biol. Cell 19: 2328-2338 (2008)
  • Miyoshi T, Tsuji T, Higashida C, Hertzog M, Fujita A, Narumiya S, Scita G, Watanabe N. Actin turnover-dependent fast dissociation of capping protein in the dendritic nucleation actin network: evidence of frequent filament severing. J. Cell Biol. 175: 947-955 (2006)
  • Watanabe, N. and Higashida, C. Formins: Processive cappers of growing actin filaments. (Review) Exp. Cell Res. 301: 16-22, (2004)
  • Higashida C. Miyoshi T., Fujita A., Oceguera-Yanez F., Monypenny J., Andou Y., Narumiya S. and Watanabe N. Actin polymerization-driven molecular movement of mDia1 in living cells. Science 303: 2007-2010 (2004)
  • Watanabe N. and Mitchison T.J. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 295: 1083-1086 (2002)
  • Kato, T., Watanabe, N., Morishima, Y., Fujita, A., Ishizaki, T. and Narumiya, S. Localization of a mammalian homolog of diaphanous, mDia1, to the mitotic spindle in HeLa cells. J. Cell Sci. 114: 775-784 (2001)
  • Watanabe N, Kato T, Fujita A, Ishizaki T, Narumiya S. Cooperation between mDia1 and ROCK in Rho-induced actin reorganization. Nat. Cell Biol. 1: 136-143 (1999)
  • Watanabe, N., Madaule, P., Reid, T., Ishizaki, T., Watanabe, G., Kakizuka, A., Saito, Y., Nakao, K., Jockusch, B.M. and Narumiya, S. p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin. EMBO J. 16: 3044-3056 (1997)
Tohoku University> Graduate School of Life Sciences> Department of Biomolecular Sciences